标签蛋白抗体

HA-Tag Mouse mAb(1B10)

 

Cat#： TDY003   |   Source： Mouse   |   Type： Monoclonal   |
Applications: WB, IP, IF   Reactivity: N/A
MW.(KDa): N/A Isotype: IgG1

Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation

Reactivity:    H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
 
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection. 
Applications Specifications Alternative Names

 

IP

 

IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with HA-tag fusion protein

3、IP(untransfted 293+anti-HA mAb+Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-HA mAb+ Protein G agarose)

6、IP (transfected 293+Protein G)

7、Recombinant protein (E.coli)


IF

 

IF analysis of 293 cells transfected with a HA-tag protein, using TDYbio anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)

 

 

Western Blot

 

0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.

 

 

Size:
TDY003C-50μL ￥800
TDY003F-100μL ￥1500
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